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Journal of Biomedical Optics

En face Doppler total retinal blood flow measurement with 70 kHz spectral optical coherence tomography
Author(s): Ou Tan; Gangjun Liu; Liu Liang; Simon S. Gao; Alex D. Pechauer; Yali Jia; David Huang
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Paper Abstract

An automated algorithm was developed for total retinal blood flow (TRBF) using 70-kHz spectral optical coherence tomography (OCT). The OCT was calibrated for the transformation from Doppler shift to speed based on a flow phantom. The TRBF scan pattern contained five repeated volume scans (2×2  mm) obtained in 3 s and centered on central retinal vessels in the optic disc. The TRBF was calculated using an en face Doppler technique. For each retinal vein, blood flow was measured at an optimal plane where the calculated flow was maximized. The TRBF was calculated by summing flow in all veins. The algorithm tracked vascular branching so that either root or branch veins are summed, but never both. The TRBF in five repeated volumes were averaged to reduce variation due to cardiac cycle pulsation. Finally, the TRBF was corrected for eye length variation. Twelve healthy eyes and 12 glaucomatous eyes were enrolled to test the algorithm. The TRBF was 45.4±6.7  μl/min for healthy control and 34.7±7.6  μl/min for glaucomatous participants (p-value=0.01). The intravisit repeatability was 8.6% for healthy controls and 8.4% for glaucoma participants. The proposed automated method provided repeatable TRBF measurement.

Paper Details

Date Published: 10 June 2015
PDF: 8 pages
J. Biomed. Opt. 20(6) 066004 doi: 10.1117/1.JBO.20.6.066004
Published in: Journal of Biomedical Optics Volume 20, Issue 6
Show Author Affiliations
Ou Tan, Casey Eye Institute (United States)
Oregon Health and Science Univ. (United States)
Gangjun Liu, Oregon Health and Science Univ. (United States)
Liu Liang, Oregon Health & Science Univ. (United States)
Simon S. Gao, Oregon Health & Science Univ. (United States)
Alex D. Pechauer, Oregon Health & Science Univ. (United States)
Yali Jia, Casey Eye Institute (United States)
Oregon Health and Science Univ. (United States)
David Huang, Casey Eye Institute (United States)
Oregon Health and Science Univ. (United States)


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