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Journal of Biomedical Optics

Label-free and depth resolved optical sectioning of iron-complex deposits in sickle cell disease splenic tissue by multiphoton microscopy
Author(s): Genevieve D. Vigil; Alexander J. Adami; Tahsin Ahmed; Aamir Khan; Sarah Chapman; Biree Andemariam; Roger S. Thrall; Scott S. Howard
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Paper Abstract

Multiphoton microscopy (MPM) imaging of intrinsic two-photon excited fluorescence (TPEF) is performed on humanized sickle cell disease (SCD) mouse model splenic tissue. Distinct morphological and spectral features associated with SCD are identified and discussed in terms of diagnostic relevance. Specifically, spectrally unique splenic iron-complex deposits are identified by MPM; this finding is supported by TPEF spectroscopy and object size to standard histopathological methods. Further, iron deposits are found at higher concentrations in diseased tissue than in healthy tissue by all imaging methods employed here including MPM, and therefore, may provide a useful biomarker related to the disease state. These newly characterized biomarkers allow for further investigations of SCD in live animals as a means to gain insight into the mechanisms impacting immune dysregulation and organ malfunction, which are currently not well understood.

Paper Details

Date Published: 4 June 2015
PDF: 8 pages
J. Biomed. Opt. 20(6) 066001 doi: 10.1117/1.JBO.20.6.066001
Published in: Journal of Biomedical Optics Volume 20, Issue 6
Show Author Affiliations
Genevieve D. Vigil, Univ. of Notre Dame (United States)
Alexander J. Adami, Univ. of Connecticut Health Ctr. (United States)
Tahsin Ahmed, Univ. of Notre Dame (United States)
Aamir Khan, Univ. of Notre Dame (United States)
Sarah Chapman, Univ. of Notre Dame (United States)
Biree Andemariam, Univ. of Connecticut Health Ctr. (United States)
Roger S. Thrall, Univ. of Connecticut Health Ctr. (United States)
Scott S. Howard, Univ. of Notre Dame (United States)

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