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Journal of Biomedical Optics

Raman spectroscopy of human skin: looking for a quantitative algorithm to reliably estimate human age
Author(s): Giuseppe Pezzotti; Marco Boffelli; Daisuke Miyamori; Takeshi Uemura; Yoshinori Marunaka; Wenliang Zhu; Hiroshi Ikegaya
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Paper Abstract

The possibility of examining soft tissues by Raman spectroscopy is challenged in an attempt to probe human age for the changes in biochemical composition of skin that accompany aging. We present a proof-of-concept report for explicating the biophysical links between vibrational characteristics and the specific compositional and chemical changes associated with aging. The actual existence of such links is then phenomenologically proved. In an attempt to foster the basics for a quantitative use of Raman spectroscopy in assessing aging from human skin samples, a precise spectral deconvolution is performed as a function of donors’ ages on five cadaveric samples, which emphasizes the physical significance and the morphological modifications of the Raman bands. The outputs suggest the presence of spectral markers for age identification from skin samples. Some of them appeared as authentic “biological clocks” for the apparent exactness with which they are related to age. Our spectroscopic approach yields clear compositional information of protein folding and crystallization of lipid structures, which can lead to a precise identification of age from infants to adults. Once statistically validated, these parameters might be used to link vibrational aspects at the molecular scale for practical forensic purposes.

Paper Details

Date Published: 25 June 2015
PDF: 18 pages
J. Biomed. Opt. 20(6) 065008 doi: 10.1117/1.JBO.20.6.065008
Published in: Journal of Biomedical Optics Volume 20, Issue 6
Show Author Affiliations
Giuseppe Pezzotti, Kyoto Institute of Technology (Japan)
Loma Linda Univ. (United States)
Osaka Univ. (Japan)
Marco Boffelli, Kyoto Institute of Technology (Japan)
Daisuke Miyamori, Kyoto Prefectural Univ. of Medicine (Japan)
Takeshi Uemura, Kyoto Prefectural Univ. of Medicine (Japan)
Yoshinori Marunaka, Kyoto Prefectural Univ. of Medicine (Japan)
Wenliang Zhu, Osaka Univ. (Japan)
Hiroshi Ikegaya, Kyoto Prefectural Univ. of Medicine (Japan)


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