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Journal of Biomedical Optics • Open Access

Label-free imaging and quantitative chemical analysis of Alzheimer’s disease brain samples with multimodal multiphoton nonlinear optical microspectroscopy
Author(s): Jang Hyuk Lee; Dae Hwan Kim; Woo Keun Song; Myoung-Kyu Oh; Do-Kyeong Ko

Paper Abstract

We developed multimodal multiphoton microspectroscopy using a small-diameter probe with gradient-index lenses and applied it to unstained Alzheimer’s disease (AD) brain samples. Our system maintained the image quality and spatial resolution of images obtained using an objective lens of similar numerical aperture. Multicolor images of AD brain samples were obtained simultaneously by integrating two-photon excited fluorescence and second-harmonic generation on a coherent anti-Stokes Raman scattering (CARS) microendoscope platform. Measurements of two hippocampal regions, the cornus ammonis-1 and dentate gyrus, revealed more lipids, amyloid fibers, and collagen in the AD samples than in the normal samples. Normal and AD brains were clearly distinguished by a large spectral difference and quantitative analysis of the CH mode using CARS microendoscope spectroscopy. We expect this system to be an important diagnosis tool in AD research.

Paper Details

Date Published: 28 May 2015
PDF: 7 pages
J. Biomed. Opt. 20(5) 056013 doi: 10.1117/1.JBO.20.5.056013
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Jang Hyuk Lee, Gwangju Institute of Science and Technology (Germany)
Dae Hwan Kim, Gwangju Institute of Science and Technology (Republic of Korea)
Woo Keun Song, Gwangju Institute of Science and Technology (Republic of Korea)
Myoung-Kyu Oh, Advanced Photonics Research Institute (Republic of Korea)
Do-Kyeong Ko, Gwangju Institute of Science and Technology (Republic of Korea)

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