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Journal of Biomedical Optics

Ex vivo characterization of normal and adenocarcinoma colon samples by Mueller matrix polarimetry
Author(s): Iftikhar Ahmad; Manzoor Ahmad; Karim Khan; Sumara Ashraf; Shakil Ahmad; Masroor Ikram
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Paper Abstract

Mueller matrix polarimetry along with polar decomposition algorithm was employed for the characterization of ex vivo normal and adenocarcinoma human colon tissues by polarized light in the visible spectral range (425–725 nm). Six derived polarization metrics [total diattenuation (DT), retardance (RT), depolarization (ΔT), linear diattenuation (DL), retardance (δ), and depolarization (ΔL)] were compared for normal and adenocarcinoma colon tissue samples. The results show that all six polarimetric properties for adenocarcinoma samples were significantly higher as compared to the normal samples for all wavelengths. The Wilcoxon rank sum test illustrated that total retardance is a good candidate for the discrimination of normal and adenocarcinoma colon samples. Support vector machine classification for normal and adenocarcinoma based on the four polarization properties spectra (ΔT, ΔL, RT,and δ) yielded 100% accuracy, sensitivity, and specificity, while both DT and DL  showed 66.6%, 33.3%, and 83.3% accuracy, sensitivity, and specificity, respectively. The combination of polarization analysis and given classification methods provides a framework to distinguish the normal and cancerous tissues.

Paper Details

Date Published: 28 May 2015
PDF: 8 pages
J. Biomed. Opt. 20(5) 056012 doi: 10.1117/1.JBO.20.5.056012
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Iftikhar Ahmad, Pakistan Institute of Engineering and Applied Sciences (Pakistan)
Ctr. for Nuclear Medicine and Radiotherapy (CENAR) (Pakistan)
Manzoor Ahmad, Pakistan Institute of Engineering and Applied Sciences (Pakistan)
Islamia College (Pakistan)
Karim Khan, Pakistan Institute of Engineering and Applied Sciences (Pakistan)
Sumara Ashraf, Pakistan Institute of Engineering and Applied Sciences (Pakistan)
Shakil Ahmad, Swat Institute of Nuclear Medicine, Oncology and Radiotherapy (Pakistan)
Masroor Ikram, Pakistan Institute of Engineering and Applied Sciences (Pakistan)


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