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Journal of Biomedical Optics • Open Access

Rare-earth-doped nanophosphors for multicolor cathodoluminescence nanobioimaging using scanning transmission electron microscopy
Author(s): Taichi Furukawa; Shoichiro Fukushima; Hirohiko Niioka; Naoki Yamamoto; Jun Miyake; Tsutomu Araki; Mamoru Hashimoto

Paper Abstract

We describe rare-earth-doped nanophosphors (RE-NPs) for biological imaging using cathodoluminescence (CL) microscopy based on scanning transmission electron microscopy (STEM). We report the first demonstration of multicolor CL nanobioimaging using STEM with nanophosphors. The CL spectra of the synthesized nanophosphors (Y2O3:Eu, Y2O3:Tb) were sufficiently narrow to be distinguished. From CL images of RE-NPs on an elastic carbon-coated copper grid, the spatial resolution was beyond the diffraction limit of light. Y2O3:Tb and Y2O3:Eu RE-NPs showed a remarkable resistance against electron beam exposure even at high acceleration voltage (80 kV) and retained a CL intensity of more than 97% compared with the initial intensity for 1 min. In biological CL imaging with STEM, heavy-metal-stained cell sections containing the RE-NPs were prepared, and both the CL images of RE-NPs and cellular structures, such as mitochondria, were clearly observed from STEM images with high contrast. The cellular CL imaging using RE-NPs also had high spatial resolution even though heavy-metal-stained cells are normally regarded as highly scattering media. Moreover, since the RE-NPs exhibit photoluminescence (PL) excited by UV light, they are useful for multimodal correlative imaging using CL and PL.

Paper Details

Date Published: 22 May 2015
PDF: 6 pages
J. Biomed. Opt. 20(5) 056007 doi: 10.1117/1.JBO.20.5.056007
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Taichi Furukawa, Osaka Univ. (Japan)
Shoichiro Fukushima, Osaka Univ. (Japan)
Hirohiko Niioka, Osaka Univ. (Japan)
Naoki Yamamoto, Tokyo Institute of Technology (Japan)
Jun Miyake, Osaka Univ. (Japan)
Tsutomu Araki, Osaka Univ. (Japan)
Mamoru Hashimoto, Osaka Univ. (Japan)

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