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Journal of Biomedical Optics • Open Access

Quantitative evaluation of in vivo vital-dye fluorescence endoscopic imaging for the detection of Barrett’s-associated neoplasia
Author(s): Nadhi Thekkek; Michelle H. Lee; Alexandros D. Polydorides; Daniel G. Rosen; Sharmila Anandasabapathy; Rebecca Richards-Kortum

Paper Abstract

Current imaging tools are associated with inconsistent sensitivity and specificity for detection of Barrett’s-associated neoplasia. Optical imaging has shown promise in improving the classification of neoplasia in vivo. The goal of this pilot study was to evaluate whether in vivo vital dye fluorescence imaging (VFI) has the potential to improve the accuracy of early-detection of Barrett’s-associated neoplasia. In vivo endoscopic VFI images were collected from 65 sites in 14 patients with confirmed Barrett’s esophagus (BE), dysplasia, or esophageal adenocarcinoma using a modular video endoscope and a high-resolution microendoscope (HRME). Qualitative image features were compared to histology; VFI and HRME images show changes in glandular structure associated with neoplastic progression. Quantitative image features in VFI images were identified for objective image classification of metaplasia and neoplasia, and a diagnostic algorithm was developed using leave-one-out cross validation. Three image features extracted from VFI images were used to classify tissue as neoplastic or not with a sensitivity of 87.8% and a specificity of 77.6% (AUC=0.878). A multimodal approach incorporating VFI and HRME imaging can delineate epithelial changes present in Barrett’s-associated neoplasia. Quantitative analysis of VFI images may provide a means for objective interpretation of BE during surveillance.

Paper Details

Date Published: 7 May 2015
PDF: 10 pages
J. Biomed. Opt. 20(5) 056002 doi: 10.1117/1.JBO.20.5.056002
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Nadhi Thekkek, Rice Univ. (United States)
Michelle H. Lee, Mount Sinai School of Medicine (United States)
Alexandros D. Polydorides, Icahn School of Medicine (United States)
Daniel G. Rosen, Baylor College of Medicine (United States)
Sharmila Anandasabapathy, Baylor College of Medicine (United States)
Rebecca Richards-Kortum, Rice Univ. (United States)


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