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Journal of Biomedical Optics • Open Access

Image distortion and its correction in linear galvanometric mirrors–based laser-scanning microscopy
Author(s): Wenbo Wang; Zhenguo Wu; Haishan Zeng

Paper Abstract

To simplify imaging focusing and calibration tasks, a laser-scanning microscope needs to scan at a moderate frame rate. The inertia of a galvanometric scanner leads to time delays when following external commands, which subsequently introduces image distortions that deteriorate as scan frequency increases. Sinusoidal and triangular waveforms were examined as fast axis driving patterns. The interplay among driving pattern, frequency, sampling rate, phase shift, linear scanning range, and their effect on reconstructed images was discussed. Utilizing position feedback from the linear galvo scanners, the effect of response time could be automatically compensated in real time. Precompensated triangular driving waveform offered the least amount of image distortion.

Paper Details

Date Published: 5 May 2015
PDF: 4 pages
J. Biomed. Opt. 20(5) 056001 doi: 10.1117/1.JBO.20.5.056001
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Wenbo Wang, The Univ. of British Columbia (Canada)
The BC Cancer Agency Research Ctr. (Canada)
Zhenguo Wu, The Univ. of British Columbia (Canada)
The BC Cancer Agency Research Ctr. (Canada)
Haishan Zeng, The Univ. of British Columbia (Canada)
The BC Cancer Agency Research Ctr. (Canada)


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