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Journal of Biomedical Optics

Raman spectroscopic study on the excystation process in a single unicellular organism amoeba (<italic<Acanthamoeba polyphaga</italic<)
Author(s): Yu-Chung Lin; Elena Perevedentseva; Chia-Liang Cheng
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Paper Abstract

An <italic<in vivo</italic< Raman spectroscopic study of amoeba (<italic<Acanthamoeba polyphaga</italic<) is presented. The changes of the spectra during the amoeba cyst activation and excystation are analyzed. The spectra show the changes of the relative intensities of bands corresponding to protein, lipid, and carotenoid components during cyst activation. The presence of carotenoids in the amoeba is observed via characteristic Raman bands. These signals in the Raman spectra are intense in cysts but decrease in intensity with cyst activation and exhibit a correlation with the life cycle of amoeba. This work demonstrates the feasibility of using Raman spectroscopy for the detection of single amoeba microorganisms <italic<in vivo</italic< and for the analysis of the amoeba life activity. The information obtained may have implications for the estimation of epidemiological situations and for the diagnostics and prognosis of the development of amoebic inflammations.

Paper Details

Date Published: 30 April 2015
PDF: 7 pages
J. Biomed. Opt. 20(5) 051042 doi: 10.1117/1.JBO.20.5.051042
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Yu-Chung Lin, National Dong Hwa Univ. (Taiwan)
Elena Perevedentseva, P.N. Lebedev Physical Institute (Taiwan)
National Dong Hwa Univ. (Russia)
Chia-Liang Cheng, National Dong Hwa Univ. (Taiwan)

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