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Journal of Biomedical Optics • Open Access

Use of time-resolved fluorescence spectroscopy to evaluate diagnostic value of collagen degradation products
Author(s): Joanna Sikora; Michał Cyrankiewicz; Tomasz Wybranowski; Blanka Ziomkowska; Borys Ośmiałowski; Ewa Obońska; Beata Augustyńska; Stefan Kruszewski; Jacek Kubica

Paper Abstract

The concentration of collagen degradation products (CDPs) may reflect the process of left ventricular remodeling (LVR). The aim of this study was to evaluate the potential diagnostic usefulness of time-resolved fluorescence spectroscopy (TRFS) in assessment of CDPs. The preliminary experiment was designed to establish if CDPs’ characteristics might be visible by mean fluorescence lifetime (FLT) in determined conditions. The in vitro model of CDPs was prepared by conducting the hydrolysis of type III collagen. The FLT of samples was measured by the time-resolved spectrometer Life Spec II with the subnanosecond pulsed 360-nm EPLED diode. The FLTs were obtained by deconvolution analysis of the data using a multiexponential model of fluorescence decay. In order to determine the limit of traceability of CDPs, a comparison of different collagen/plasma ratio in samples was performed. The results of our study showed that the increase of added plasma to hydrolyzed collagen extended the mean FLT. Thus, the diagnosis of LVR based on measurements using TRFS is possible. However, it is important to point out the experiment was preliminary and further investigation in this field of research is crucial.

Paper Details

Date Published: 12 March 2015
PDF: 8 pages
J. Biomed. Opt. 20(5) 051039 doi: 10.1117/1.JBO.20.5.051039
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Joanna Sikora, Collegium Medicum UMK (Poland)
Michał Cyrankiewicz, Collegium Medicum UMK (Poland)
Tomasz Wybranowski, Collegium Medicum UMK (Poland)
Blanka Ziomkowska, Collegium Medicum UMK (Poland)
Borys Ośmiałowski, Univ. of Technology and Life Sciences (Poland)
Ewa Obońska, Collegium Medicum UMK (Poland)
Beata Augustyńska, Collegium Medicum UMK (Poland)
Stefan Kruszewski, Collegium Medicum UMK (Poland)
Jacek Kubica, Collegium Medicum UMK (Poland)

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