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Journal of Biomedical Optics

Identification of individual biofilm-forming bacterial cells using Raman tweezers
Author(s): Ota Samek; Silvie Bernatová; Jan Ježek; Martin Šiler; Mojmir Šerý; Vladislav Krzyzanek; Kamila Hrubanová; Pavel Zemánek; Veronika Holá; Filip Ruzicka
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Paper Abstract

A method for <italic<in vitro</italic< identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral “Raman fingerprints” obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilm-negative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of <italic<Staphylococcus epidermidis</italic<. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment.

Paper Details

Date Published: 2 March 2015
PDF: 6 pages
J. Biomed. Opt. 20(5) 051038 doi: 10.1117/1.JBO.20.5.051038
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Ota Samek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Silvie Bernatová, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Jan Ježek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Martin Šiler, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Mojmir Šerý, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Vladislav Krzyzanek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Kamila Hrubanová, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Pavel Zemánek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Veronika Holá, Masaryk Univ. (Czech Republic)
Filip Ruzicka, Masaryk Univ. (Czech Republic)


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