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Journal of Biomedical Optics

Noninvasive glucose sensing by transcutaneous Raman spectroscopy
Author(s): Wei-Chuan Shih; Kate L. Bechtel; Mihailo V. Rebec
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Paper Abstract

We present the development of a transcutaneous Raman spectroscopy system and analysis algorithm for noninvasive glucose sensing. The instrument and algorithm were tested in a preclinical study in which a dog model was used. To achieve a robust glucose test system, the blood levels were clamped for periods of up to 45 min. Glucose clamping and rise/fall patterns have been achieved by injecting glucose and insulin into the ear veins of the dog. Venous blood samples were drawn every 5 min and a plasma glucose concentration was obtained and used to maintain the clamps, to build the calibration model, and to evaluate the performance of the system. We evaluated the utility of the simultaneously acquired Raman spectra to be used to determine the plasma glucose values during the 8-h experiment. We obtained prediction errors in the range of ∼1.5−2  mM. These were in-line with a best-case theoretical estimate considering the limitations of the signal-to-noise ratio estimates. As expected, the transition regions of the clamp study produced larger predictive errors than the stable regions. This is related to the divergence of the interstitial fluid (ISF) and plasma glucose values during those periods. Two key contributors to error beside the ISF/plasma difference were photobleaching and detector drift. The study demonstrated the potential of Raman spectroscopy in noninvasive applications and provides areas where the technology can be improved in future studies.

Paper Details

Date Published: 17 February 2015
PDF: 5 pages
J. Biomed. Opt. 20(5) 051036 doi: 10.1117/1.JBO.20.5.051036
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Wei-Chuan Shih, Univ. of Houston (United States)
University of Houston (United States)
Spectroscopy Laboratory (United States)
Kate L. Bechtel, Massachusetts Institute of Technology (United States)
Mihailo V. Rebec, iSense CGM (United States)

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