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Journal of Biomedical Optics

Multidimensional visualization of healthy and sensitized rabbit knee tissues by means of confocal microscopy
Author(s): Romualdas Rudys; Saulius Bagdonas; Gailutė Kirdaitė; Jurga Papečkienė; Ričardas Rotomskis
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Paper Abstract

This study combines several fluorescence detection methods to distinguish structural features of the synovium and cartilage tissues and to visualize the localization of endogenous porphyrins in the sensitized tissues. Specimens of synovium and cartilage tissues obtained from rabbits with antigen-induced monoarthritis after intra-articular 5-aminolevulinic acid methyl ester injection and those from healthy rabbits were investigated <italic<ex vivo</italic< by means of fluorescence spectroscopy, fluorescence intensity, and lifetime microscopy. The presence of endogenous porphyrins was confirmed with the fluorescence spectra measured on sliced sensitized specimens. Application of the lifetime-gating method on fast fluorescence lifetime imaging microscopy images, allowed separate visualization of tissue structures possessing different average lifetimes. The presence of the structures has been validated by histopathological imaging based on conventional rapid hematoxylin–eosin staining of the specimens. The fluorescence lifetime of endogenous protoporphyrin IX has been assessed and employed for visualization of sensitized tissues.

Paper Details

Date Published: 11 February 2015
PDF: 9 pages
J. Biomed. Opt. 20(5) 051035 doi: 10.1117/1.JBO.20.5.051035
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Romualdas Rudys, The National Cancer Institute (Lithuania)
State Scientific Research Institute of Innovative Medical Ctr. (Lithuania)
Saulius Bagdonas, Vilnius Univ. (Lithuania)
Gailutė Kirdaitė, State Scientific Research Institute of Innovative Medical Ctr. (Lithuania)
Jurga Papečkienė, State Scientific Research Institute of Innovative Medical Ctr. (Lithuania)
Ričardas Rotomskis, The National Cancer Institute (Lithuania)


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