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Journal of Biomedical Optics

Photobleaching effects on in vivo skin autofluorescence lifetime
Author(s): Inesa Ferulova; Alexey Lihachev; Janis Spigulis
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Paper Abstract

The autofluorescence lifetime of healthy human skin was measured using excitation provided by a picosecond diode laser operating at a wavelength of 405 nm and with fluorescence emission collected at 475 and 560 nm. In addition, spectral and temporal responses of healthy human skin and intradermal nevus in the spectral range 460 to 610 nm were studied before and after photobleaching. A decrease in the autofluorescences lifetimes changes was observed after photobleaching of human skin. A three-exponential model was used to fit the signals, and under this model, the most significant photoinduced changes were observed for the slowest lifetime component in healthy skin at the spectral range 520 to 610 nm and intradermal nevus at the spectral range 460 to 610 nm.

Paper Details

Date Published: 30 January 2015
PDF: 5 pages
J. Biomed. Opt. 20(5) 051031 doi: 10.1117/1.JBO.20.5.051031
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Inesa Ferulova, Univ. of Latvia (Latvia)
Alexey Lihachev, Univ. of Latvia (Latvia)
Janis Spigulis, Univ. of Latvia (Latvia)

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