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Journal of Biomedical Optics

Measuring the dynamics of cyclic adenosine monophosphate level in living cells induced by low-level laser irradiation using bioluminescence resonance energy transfer
Author(s): Yimei Huang; Liqin Zheng; Hongqin Yang; Jiangxu Chen; Yuhua Wang; Hui Li; Shusen Xie; Haishan Zeng
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Paper Abstract

Several studies demonstrated that the cyclic adenosine monophosphate (cAMP), an important second messenger, is involved in the mechanism of low-level laser irradiation (LLLI) treatment. However, most of these studies obtained the cAMP level in cell culture extracts or supernatant. In this study, the cAMP level in living cells was measured with bioluminescence resonance energy transfer (BRET). The effect of LLLI on cAMP level in living cells with adenosine receptors blocked was explored to identify the role of adenosine receptors in LLLI. The results showed that LLLI increased the cAMP level. Moreover, the rise of cAMP level was light dose dependent but wavelength independent for 658-, 785-, and 830-nm laser light. The results also exhibited that the adenosine receptors, a class of G protein-coupled receptor (GPCR), modulated the increase of cAMP level induced by LLLI. The cAMP level increased more significantly when the A3 adenosine receptors (A3R) were blocked by A3R antagonist compared with A1 adenosine receptor or A2a adenosine receptor blocked in HEK293T cells after LLLI, which was in good agreement with the adenosine receptors’ expressions. All these results suggested that measuring the cAMP level with BRET could be a useful technique to study the role of GPCRs in living cells under LLLI.

Paper Details

Date Published: 22 January 2015
PDF: 6 pages
J. Biomed. Opt. 20(5) 051029 doi: 10.1117/1.JBO.20.5.051029
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Yimei Huang, Fujian Normal Univ. (China)
The BC Cancer Agency Research Ctr. (Canada)
Liqin Zheng, Fujian Normal Univ. (China)
Hongqin Yang, Fujian Normal Univ. (China)
Jiangxu Chen, Fujian Normal Univ. (China)
Yuhua Wang, Fujian Normal Univ. (China)
Hui Li, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)
Haishan Zeng, The BC Cancer Agency Research Ctr. (Canada)

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