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Journal of Biomedical Optics • Open Access

Multispectral imaging of absorption and scattering properties of in vivo exposed rat brain using a digital red-green-blue camera
Author(s): Keiichiro Yoshida; Izumi Nishidate; Tomohiro Ishizuka; Satoko Kawauchi; Shunichi Sato; Manabu Sato

Paper Abstract

n order to estimate multispectral images of the absorption and scattering properties in the cerebral cortex of in vivo rat brain, we investigated spectral reflectance images estimated by the Wiener estimation method using a digital RGB camera. A Monte Carlo simulation-based multiple regression analysis for the corresponding spectral absorbance images at nine wavelengths (500, 520, 540, 560, 570, 580, 600, 730, and 760 nm) was then used to specify the absorption and scattering parameters of brain tissue. In this analysis, the concentrations of oxygenated hemoglobin and that of deoxygenated hemoglobin were estimated as the absorption parameters, whereas the coefficient a and the exponent b of the reduced scattering coefficient spectrum approximated by a power law function were estimated as the scattering parameters. The spectra of absorption and reduced scattering coefficients were reconstructed from the absorption and scattering parameters, and the spectral images of absorption and reduced scattering coefficients were then estimated. In order to confirm the feasibility of this method, we performed in vivo experiments on exposed rat brain. The estimated images of the absorption coefficients were dominated by the spectral characteristics of hemoglobin. The estimated spectral images of the reduced scattering coefficients had a broad scattering spectrum, exhibiting a larger magnitude at shorter wavelengths, corresponding to the typical spectrum of brain tissue published in the literature. The changes in the estimated absorption and scattering parameters during normoxia, hyperoxia, and anoxia indicate the potential applicability of the method by which to evaluate the pathophysiological conditions of in vivo brain due to the loss of tissue viability.

Paper Details

Date Published: 23 January 2015
PDF: 15 pages
J. Biomed. Opt. 20(5) 051026 doi: 10.1117/1.JBO.20.5.051026
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Keiichiro Yoshida, Tokyo Univ. of Agriculture and Technology (Japan)
Izumi Nishidate, Tokyo Univ. of Agriculture and Technology (Japan)
Tomohiro Ishizuka, Tokyo Univ. of Agriculture and Technology (Japan)
Satoko Kawauchi, National Defense Medical College (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Manabu Sato, Yamagata Univ. (Japan)

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