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Journal of Biomedical Optics • Open Access

Second-harmonic generation scattering directionality predicts tumor cell motility in collagen gels
Author(s): Kathleen A. Burke; Ryan P. Dawes; Mehar K. Cheema; Amy Van Hove; Danielle S. W. Benoit; Seth W. Perry; Edward Brown

Paper Abstract

Second-harmonic generation (SHG) allows for the analysis of tumor collagen structural changes throughout metastatic progression. SHG directionality, measured through the ratio of the forward-propagating to backward-propagating signal (F/B ratio), is affected by collagen fibril diameter, spacing, and disorder of fibril packing within a fiber. As tumors progress, these parameters evolve, producing concurrent changes in F/B. It has been recently shown that the F/B of highly metastatic invasive ductal carcinoma (IDC) breast tumors is significantly different from less metastatic tumors. This suggests a possible relationship between the microstructure of collagen, as measured by the F/B, and the ability of tumor cells to locomote through that collagen. Utilizing

Paper Details

Date Published: 27 January 2015
PDF: 9 pages
J. Biomed. Opt. 20(5) 051024 doi: 10.1117/1.JBO.20.5.051024
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Kathleen A. Burke, Univ. of Rochester (United States)
Ryan P. Dawes, Univ. of Rochester Medical Ctr. (United States)
Mehar K. Cheema, Stony Brook Univ. (United States)
Amy Van Hove, Univ. of Rochester (United States)
Danielle S. W. Benoit, Univ. of Rochester (United States)
Seth W. Perry, Univ. of Rochester (United States)
Edward Brown, Univ. of Rochester Medical Ctr. (United States)


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