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Journal of Biomedical Optics

Combination of low level light therapy and nitrosyl-cobinamide accelerates wound healing
Author(s): Ryan Spitler; Hsiang Ho; Frederique Norpetlian; Xiangduo Kong; Jingjing Jiang; Kyoko Yokomori; Bogi Andersen; Gerry R. Boss; Michael W. Berns
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Paper Abstract

Low level light therapy (LLLT) has numerous therapeutic benefits, including improving wound healing, but the precise mechanisms involved are not well established; in particular, the underlying role of cytochrome C oxidase (C-ox) as the primary photoacceptor and the associated biochemical mechanisms still require further investigation. We previously showed the nitric oxide (NO) donating drug nitrosyl-cobinamide (NO-Cbi) enhances wound healing through a cGMP/cGMP-dependent protein kinase/ERK1/2 mechanism. Here, we show that the combination of LLLT and NO-Cbi markedly improves wound healing compared to either treatment alone. LLLT-enhanced wound healing proceeded through an electron transport chain-C-ox-dependent mechanism with a reduction of reactive oxygen species and increased adenosine triphosphate production. C-ox was validated as the primary photoacceptor by three observations: increased oxygen consumption, reduced wound healing in the presence of sodium azide, and disassociation of cyanide, a known C-ox ligand, following LLLT. We conclude that LLLT and NO-Cbi accelerate wound healing through two independent mechanisms, the electron transport chain-C-ox pathway and cGMP signaling, respectively, with both resulting in ERK1/2 activation.

Paper Details

Date Published: 6 January 2015
PDF: 8 pages
J. Biomed. Opt. 20(5) 051022 doi: 10.1117/1.JBO.20.5.051022
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Ryan Spitler, Univ. of California, Irvine (United States)
Hsiang Ho, Univ. of California, Irvine (United States)
Frederique Norpetlian, Univ. of California, Irvine (United States)
Xiangduo Kong, Univ. of California, Irvine (United States)
Jingjing Jiang, Univ. of California, San Diego (United States)
Kyoko Yokomori, Univ. of California, Irvine (United States)
Bogi Andersen, Univ. of California, Irvine (United States)
Gerry R. Boss, Univ. of California, San Diego (United States)
Michael W. Berns, Univ. of California, Irvine (United States)
Univ. of California, San Diego (United States)

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