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Journal of Biomedical Optics

Optical coherence tomography guided microinjections in live mouse embryos: high-resolution targeted manipulation for mouse embryonic research
Author(s): Saba H. Syed; Andrew J. Coughlin; Monica D. Garcia; Shang Wang; Jennifer L. West; Kirill V. Larin; Irina V. Larina
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Paper Abstract

The ability to conduct highly localized delivery of contrast agents, viral vectors, therapeutic or pharmacological agents, and signaling molecules or dyes to live mammalian embryos is greatly desired to enable a variety of studies in the field of developmental biology, such as investigating the molecular regulation of cardiovascular morphogenesis. To meet such a demand, we introduce, for the first time, the concept of employing optical coherence tomography (OCT)-guide microinjections in live mouse embryos, which provides precisely targeted manipulation with spatial resolution at the micrometer scale. The feasibility demonstration is performed with experimental studies on cultured live mouse embryos at E8.5 and E9.5. Additionally, we investigate the OCT-guided microinjection of gold–silica nanoshells to the yolk sac vasculature of live cultured mouse embryos at the stage when the heart just starts to beat, as a potential approach for dynamic assessment of cardiovascular form and function before the onset of blood cell circulation. Also, the capability of OCT to quantitatively monitor and measure injection volume is presented. Our results indicate that OCT-guided microinjection could be a useful tool for mouse embryonic research.

Paper Details

Date Published: 12 January 2015
PDF: 7 pages
J. Biomed. Opt. 20(5) 051020 doi: 10.1117/1.JBO.20.5.051020
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Saba H. Syed, Baylor College of Medicine (United States)
Andrew J. Coughlin, Duke Univ. (United States)
Monica D. Garcia, Baylor College of Medicine (United States)
Shang Wang, Baylor College of Medicine (United States)
Jennifer L. West, Duke Univ. (United States)
Kirill V. Larin, Baylor College of Medicine (United States)
Univ. of Houston (United States)
Irina V. Larina, Baylor College of Medicine (United States)

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