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Journal of Biomedical Optics

Diffusion characteristics of ethylene glycol in skeletal muscle
Author(s): Luís M. Oliveira; Maria Inês Carvalho; Elisabete Maria Nogueira; Valery V. Tuchin
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Paper Abstract

Part of the optical clearing study in biological tissues concerns the determination of the diffusion characteristics of water and optical clearing agents in the subject tissue. Such information is sufficient to characterize the time dependence of the optical clearing mechanisms—tissue dehydration and refractive index (RI) matching. We have used a simple method based on collimated optical transmittance measurements made from muscle samples under treatment with aqueous solutions containing different concentrations of ethylene glycol (EG), to determine the diffusion time values of water and EG in skeletal muscle. By representing the estimated mean diffusion time values from each treatment as a function of agent concentration in solution, we could identify the real diffusion times for water and agent. These values allowed for the calculation of the correspondent diffusion coefficients for those fluids. With these results, we have demonstrated that the dehydration mechanism is the one that dominates optical clearing in the first minute of treatment, while the RI matching takes over the optical clearing operations after that and remains for a longer time of treatment up to about 10 min, as we could see for EG and thin tissue samples of 0.5 mm.

Paper Details

Date Published: 19 December 2014
PDF: 10 pages
J. Biomed. Opt. 20(5) 051019 doi: 10.1117/1.JBO.20.5.051019
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Luís M. Oliveira, Instituto Politécnico do Porto (Portugal)
Univ. do Porto (Portugal)
Ctr of Innovation in Engineering & Industrial Technology (Portugal)
Maria Inês Carvalho, Univ. do Porto (Portugal)
INESC TEC (Portugal)
Elisabete Maria Nogueira, Instituto Politécnico do Porto (Portugal)
Ctr of Innovation in Engineering & Industrial Technology (Portugal)
Valery V. Tuchin, N.G. Chernyshevsky Saratov State Univ. (Russia)
Institute of Precise Mechanics & Control (Russia)
Univ. of Oulu (Finland)

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