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Journal of Biomedical Optics

Antibacterial effect of ultrafine nanodiamond against gram-negative bacteria Escherichia coli
Author(s): Anindita Chatterjee; Elena Perevedentseva; Mona Jani; Chih-Yuan Cheng; Ying-Siou Ye; Pei-Hua Chung; Chia-Liang Cheng
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Paper Abstract

We investigate the antibacterial effect of ultrafine nanodiamond particles with an average size of 5 nm against the gram-negative bacteria Escherichia coli (E. coli). UV-visible, Raman spectroscopy, and scanning electron microscopy (SEM) have been employed to elucidate the nature of the interaction. The influence on bacterial growth was monitored by measuring optical densities of E. coli at 600 nm as a function of time in the presence of carboxylated nanodiamond (cND) particles (100  μg/ml) in highly nutritious liquid Luria–Bertani medium. The SEM images prove that cND particles are attached to the bacterial cell wall surface and some portion of the bacterial cell wall undergoes destruction. Due to the change of the protein structure on the bacterial wall, a small Raman shift in the region of 1400 to 1700  cm1 was observed when E. coli interacted with cNDs. Raman mapping images show strong evidence of cND attachment at the bacterial cell wall surface. Electrotransformation of E. coli with a fluorescent protein markers experiment demonstrated that the interaction mechanisms are different for E. coli treated with cND particles, E. coli by lysozyme treatment, and E. coli that suffer lysis.

Paper Details

Date Published: 15 December 2014
PDF: 9 pages
J. Biomed. Opt. 20(5) 051014 doi: 10.1117/1.JBO.20.5.051014
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Anindita Chatterjee, National Dong Hwa Univ. (Taiwan)
Elena Perevedentseva, National Dong Hwa Univ. (Taiwan)
Mona Jani, National Dong Hwa Univ. (Taiwan)
Chih-Yuan Cheng, National Dong Hwa Univ. (Taiwan)
Ying-Siou Ye, National Dong Hwa Univ. (Taiwan)
Pei-Hua Chung, National Dong Hwa Univ. (Taiwan)
Chia-Liang Cheng, National Dong Hwa Univ. (Taiwan)

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