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Journal of Biomedical Optics

Penetration of silver nanoparticles into porcine skin <italic<ex vivo</italic< using fluorescence lifetime imaging microscopy, Raman microscopy, and surface-enhanced Raman scattering microscopy
Author(s): Yongjian Zhu; Chun-Sik Choe; Sebastian Ahlberg; Martina C. Meinke; Alexiev Ulrike; Juergen M. Lademann; Maxim E. Darvin
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Paper Abstract

In order to investigate the penetration depth of silver nanoparticles (Ag NPs) inside the skin, porcine ears treated with Ag NPs are measured by two-photon tomography with a fluorescence lifetime imaging microscopy (TPT-FLIM) technique, confocal Raman microscopy (CRM), and surface-enhanced Raman scattering (SERS) microscopy. Ag NPs are coated with poly-N-vinylpyrrolidone and dispersed in pure water solutions. After the application of Ag NPs, porcine ears are stored in the incubator for 24 h at a temperature of 37°C. The TPT-FLIM measurement results show a dramatic decrease of the Ag NPs’ signal intensity from the skin surface to a depth of 4  μm. Below 4  μm, the Ag NPs’ signal continues to decline, having completely disappeared at 12 to 14  μm depth. CRM shows that the penetration depth of Ag NPs is 11.1±2.1  μm. The penetration depth measured with a highly sensitive SERS microscopy reaches 15.6±8.3  μm. Several results obtained with SERS show that the penetration depth of Ag NPs can exceed the stratum corneum (SC) thickness, which can be explained by both penetration of trace amounts of Ag NPs through the SC barrier and by the measurements inside the hair follicle, which cannot be excluded in the experiment.

Paper Details

Date Published: 13 November 2014
PDF: 8 pages
J. Biomed. Opt. 20(5) 051006 doi: 10.1117/1.JBO.20.5.051006
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Yongjian Zhu, Charité–Univ. Berlin (Germany)
Zhejiang Univ. of Science and Technology (China)
Chun-Sik Choe, Charité Universitätsmedizin Berlin (Germany)
Kim Il Sung Univ. (Democratic People’s Republic of Korea)
Sebastian Ahlberg, Charité Universitätsmedizin Berlin (Germany)
Martina C. Meinke, Charité Universitätsmedizin Berlin (Germany)
Alexiev Ulrike, Freie Univ. Berlin (Germany)
Juergen M. Lademann, Charité Universitätsmedizin Berlin (Germany)
Maxim E. Darvin, Charité Universitätsmedizin Berlin (Germany)

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