Share Email Print
cover

Journal of Biomedical Optics

Characterization of mixtures of compounds produced in chlorpromazine aqueous solutions by ultraviolet laser irradiation: their applications in antimicrobial assays
Author(s): Tatiana Alexandru; Angela Staicu; Alexandru I. Pascu; Elena Radu; Alexandru Stoicu; Viorel V. Nastasa; Andra C. Dinache; Mihai Boni; Leonard Amaral; Mihail Lucian Pascu
Format Member Price Non-Member Price
PDF $20.00 $25.00

Paper Abstract

The study reports an investigation of the photoproducts obtained by exposure of chlorpromazine hydrochloride in ultrapure water (concentration 2  mg/mL) to a 266-nm laser beam obtained by fourth harmonic generation from a Nd:YAG laser (6-ns full time width at half maximum, 10-Hz pulse repetition rate). The photoproducts were analyzed by steady-state UV-Vis absorption, laser-induced fluorescence, Fourier transform infrared spectroscopy, and liquid chromatography–tandem time-of-flight mass spectroscopy. Two figures showing pathways that take place during irradiation for obtaining the final products are shown. The quantum yield of singlet oxygen generation by chlorpromazine (CPZ) was determined relative to standard Zn-phthalocyanine in dimethyl sulfoxide. To outline the role of fluorescence in photoproducts formation rates, fluorescence quantum yield of CPZ during exposure to 355-nm radiation (third harmonic of the fundamental beam of Nd:YAG laser) was investigated relative to standard Coumarin 1 in ethanol. The CPZ solutions exposed 60 and 240 min to 266-nm laser beam, respectively, were tested against Staphylococcus aureus ATCC 25923 strain. For 25  μL of CPZ samples irradiated 240 min, a higher diameter of inhibition has obtained against the tested strain than for the 60-min exposed ones.

Paper Details

Date Published: 3 November 2014
PDF: 11 pages
J. Biomed. Opt. 20(5) 051002 doi: 10.1117/1.JBO.20.5.051002
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Tatiana Alexandru, National Institute for Laser, Plasma and Radiation Physics (Romania)
Univ. of Bucharest (Romania)
Angela Staicu, National Institute for Laser, Plasma and Radiation Physics (Romania)
Alexandru I. Pascu, National Institute for Laser, Plasma and Radiation Physics (Romania)
Elena Radu, National Institute for Research and Development in Chemistry and Petrochemistry (Romania)
Alexandru Stoicu, National Institute for Laser, Plasma and Radiation Physics (Romania)
Univ. of Bucharest (Romania)
Viorel V. Nastasa, National Institute for Laser, Plasma and Radiation Physics (Romania)
Andra C. Dinache, National Institute for Laser, Plasma and Radiation Physics (Romania)
Mihai Boni, National Institute for Laser, Plasma and Radiation Physics (Romania)
Univ. of Bucharest (Romania)
Leonard Amaral, Instituto de Higiene e Medicina Tropical UNL (Portugal)
Univ. de Lisboa (Portugal)
Mihail Lucian Pascu, National Institute for Laser, Plasma and Radiation Physics (Romania)
Univ. of Bucharest (Romania)


© SPIE. Terms of Use
Back to Top