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Journal of Biomedical Optics • Open Access

Targeted imaging of cancer by fluorocoxib C, a near-infrared cyclooxygenase-2 probe
Author(s): Md. Jashim Uddin; Brenda C. Crews; Kebreab Ghebreselasie; Cristina K. Daniel; Philip J. Kingsley; Shu Xu; Lawrence J. Marnett

Paper Abstract

Cyclooxygenase-2 (COX-2) is a promising target for the imaging of cancer in a range of diagnostic and therapeutic settings. We report a near-infrared COX-2-targeted probe, fluorocoxib C (FC), for visualization of solid tumors by optical imaging. FC exhibits selective and potent COX-2 inhibition in both purified protein and human cancer cell lines. <italic<In vivo</italic< optical imaging shows selective accumulation of FC in COX-2-overexpressing human tumor xenografts [1483 head and neck squamous cell carcinoma (HNSCC)] implanted in nude mice, while minimal uptake is detectable in COX-2-negative tumor xenografts (HCT116) or 1483 HNSCC xenografts preblocked with the COX-2-selective inhibitor celecoxib. Time course imaging studies conducted from 3 h to 7-day post-FC injection revealed a marked reduction in nonspecific fluorescent signals with retention of fluorescence in 1483 HNSCC tumors. Thus, use of FC in a delayed imaging protocol offers an approach to improve imaging signal-to-noise that should improve cancer detection in multiple preclinical and clinical settings.

Paper Details

Date Published: 13 May 2015
PDF: 3 pages
J. Biomed. Opt. 20(5) 050502 doi: 10.1117/1.JBO.20.5.050502
Published in: Journal of Biomedical Optics Volume 20, Issue 5
Show Author Affiliations
Md. Jashim Uddin, Vanderbilt Univ. School of Medicine (United States)
Brenda C. Crews, Vanderbilt Univ. School of Medicine (United States)
Kebreab Ghebreselasie, Vanderbilt Univ. School of Medicine (United States)
Cristina K. Daniel, Vanderbilt Univ. School of Medicine (United States)
Philip J. Kingsley, Vanderbilt Univ. School of Medicine (United States)
Shu Xu, Vanderbilt Univ. School of Medicine (United States)
Lawrence J. Marnett, Vanderbilt Univ. School of Medicine (United States)


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