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Journal of Biomedical Optics • Open Access

<italic<In vivo</italic< evaluation of battery-operated light-emitting diode-based photodynamic therapy efficacy using tumor volume and biomarker expression as endpoints
Author(s): Srivalleesha Mallidi; Zhiming Mai; Imran Rizvi; Joshua Hempstead; Stephen Arnason; Jonathan P. Celli; Tayyaba Hasan

Paper Abstract

In view of the increase in cancer-related mortality rates in low- to middle-income countries (LMIC), there is an urgent need to develop economical therapies that can be utilized at minimal infrastructure institutions. Photodynamic therapy (PDT), a photochemistry-based treatment modality, offers such a possibility provided that low-cost light sources and photosensitizers are available. In this proof-of-principle study, we focus on adapting the PDT light source to a low-resource setting and compare an inexpensive, portable, battery-powered light-emitting diode (LED) light source with a standard, high-cost laser source. The comparison studies were performed <italic<in vivo</italic< in a xenograft murine model of human squamous cell carcinoma subjected to 5-aminolevulinic acid-induced protoporphyrin IX PDT. We observed virtually identical control of the tumor burden by both the LED source and the standard laser source. Further insights into the biological response were evaluated by biomarker analysis of necrosis, microvessel density, and hypoxia [carbonic anhydrase IX (CAIX) expression] among groups of control, LED-PDT, and laser-PDT treated mice. There is no significant difference in the percent necrotic volume and CAIX expression in tumors that were treated with the two different light sources. These encouraging preliminary results merit further investigations in orthotopic animal models of cancers prevalent in LMICs.

Paper Details

Date Published: 24 April 2015
PDF: 8 pages
J. Biomed. Opt. 20(4) 048003 doi: 10.1117/1.JBO.20.4.048003
Published in: Journal of Biomedical Optics Volume 20, Issue 4
Show Author Affiliations
Srivalleesha Mallidi, Harvard Medical School (United States)
Massachusetts General Hospital (United States)
Zhiming Mai, Massachusetts General Hospital (United States)
Imran Rizvi, Brigham and Women's Hospital (United States)
Massachusetts General Hospital (United States)
Joshua Hempstead, Univ. of Massachusetts Boston (United States)
Stephen Arnason, Univ. of Massachusetts Boston (United States)
Jonathan P. Celli, Univ. of Massachusetts Boston (United States)
Tayyaba Hasan, Wellman Ctr. for Photomedicine (United States)
Massachusetts General Hospital (United States)

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