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Journal of Biomedical Optics

Raman microspectroscopy of nanodiamond-induced structural changes in albumin
Author(s): Anastasiya S. Svetlakova; Nikolay N. Brandt; Alexander V. Priezzhev; Andrey Y. Chikishev
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Paper Abstract

Nanodiamonds (NDs) are promising agents for theranostic applications due to reported low toxicity and high biocompatibility, which is still being extensively tested on cellular, tissue, and organism levels. It is presumed that for experimental and future clinical applications, NDs will be administered into the organism via the blood circulation system. In this regard, the interaction of NDs with blood components needs to be thoroughly studied. We studied the interaction of carboxylated NDs (cNDs) with albumin, one of the major proteins of blood plasma. After 2-h long <italic<in vitro</italic< incubation in an aqueous solution of the protein, 100-nm cNDs were dried and the dry samples were studied with the aid of Raman microspectroscopy. The spectroscopic data indicate significant conformational changes that can be due to cND–protein interaction. A possible decrease in the functional activity of albumin related to the conformational changes must be taken into account in the <italic<in vivo</italic< applications.

Paper Details

Date Published: 22 April 2015
PDF: 5 pages
J. Biomed. Opt. 20(4) 047004 doi: 10.1117/1.JBO.20.4.047004
Published in: Journal of Biomedical Optics Volume 20, Issue 4
Show Author Affiliations
Anastasiya S. Svetlakova, Lomonosov Moscow State Univ. (Russia)
Nikolay N. Brandt, Lomonosov Moscow State University (Russia)
Alexander V. Priezzhev, Lomonosov Moscow State Univ. (Russian Federation)
Andrey Y. Chikishev, Lomonosov Moscow State Univ. (Russia)


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