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Journal of Biomedical Optics

Different Raman spectral patterns of primary rat pancreatic β cells and insulinoma cells
Author(s): Qiu-Li Zhou; Xi Rong; Fang Wei; Rui-Qiong Luo; Hong Liu
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Paper Abstract

As a noninvasive and label-free analytical technique, Raman spectroscopy has been widely used to study the difference between malignant cells and normal cells. Insulinomas are functional β-cell tumors of pancreatic islet cells. They exhibit many structural and immunohistochemical features in common with normal pancreatic β cells; thus, they are typically difficult to distinguish under the microscope, especially in vivo. We investigated insulinoma and primary rat pancreatic β-cell populations using Raman spectroscopy. The details of the optical heterogeneity between these two populations were determined based on different Raman regions primarily involving nucleic acid and protein contents, which are the most distinct cellular contents in these two types of cells. Using principal component analysis–linear discriminant analysis, these two cell types can be readily separated. The results of this work indicate that Raman spectroscopy is a promising tool for the noninvasive and label-free differentiation of insulinoma cells and normal pancreatic β cells.

Paper Details

Date Published: 7 April 2015
PDF: 6 pages
J. Biomed. Opt. 20(4) 047001 doi: 10.1117/1.JBO.20.4.047001
Published in: Journal of Biomedical Optics Volume 20, Issue 4
Show Author Affiliations
Qiu-Li Zhou, Guangxi Medical Univ. (China)
Xi Rong, Guangxi Medical Univ. (China)
Fang Wei, Guangxi Medical Univ. (China)
Rui-Qiong Luo, Guangxi Medical Univ. (China)
Hong Liu, Guangxi Medical Univ. (China)


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