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Journal of Biomedical Optics

Quantum dot imaging in the second near-infrared optical window: studies on reflectance fluorescence imaging depths by effective fluence rate and multiple image acquisition
Author(s): Yebin Jung; Sanghwa Jeong; Won Nayoun; Boeun Ahn; Jungheon Kwag; Sang Geol Kim; Sungjee Kim
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Paper Abstract

Quantum dot (QD) imaging capability was investigated by the imaging depth at a near-infrared second optical window (SOW; 1000 to 1400 nm) using time-modulated pulsed laser excitations to control the effective fluence rate. Various media, such as liquid phantoms, tissues, and in vivo small animals, were used and the imaging depths were compared with our predicted values. The QD imaging depth under excitation of continuous 20  mW/cm2 laser was determined to be 10.3 mm for 2 wt% hemoglobin phantom medium and 5.85 mm for 1 wt% intralipid phantom, which were extended by more than two times on increasing the effective fluence rate to 2000  mW/cm2. Bovine liver and porcine skin tissues also showed similar enhancement in the contrast-to-noise ratio (CNR) values. A QD sample was inserted into the abdomen of a mouse. With a higher effective fluence rate, the CNR increased more than twofold and the QD sample became clearly visualized, which was completely undetectable under continuous excitation. Multiple acquisitions of QD images and averaging process pixel by pixel were performed to overcome the thermal noise issue of the detector in SOW, which yielded significant enhancement in the imaging capability, showing up to a 1.5 times increase in the CNR.

Paper Details

Date Published: 28 April 2015
PDF: 9 pages
J. Biomed. Opt. 20(4) 046012 doi: 10.1117/1.JBO.20.4.046012
Published in: Journal of Biomedical Optics Volume 20, Issue 4
Show Author Affiliations
Yebin Jung, Pohang Univ. of Science and Technology (Republic of Korea)
Sanghwa Jeong, Pohang Univ. of Science and Technology (Republic of Korea)
Won Nayoun, Pohang Univ. of Science and Technology (Republic of Korea)
Boeun Ahn, Pohang Univ. of Science and Technology (Republic of Korea)
Jungheon Kwag, Pohang Univ. of Science and Technology (Republic of Korea)
Sang Geol Kim, Kyungpook National Univ. School of Medicine (Republic of Korea)
Sungjee Kim, Pohang Univ. of Science and Technology (Korea, Republic of)

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