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Journal of Biomedical Optics

<italic<In vitro</italic< colocalization of plasmonic nano-biolabels and biomolecules using plasmonic and Raman scattering microspectroscopy
Author(s): Kamalesh Chaudhari; Thalappil Pradeep
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Paper Abstract

An insight into the intracellular fate of theranostics is important for improving their potential in biological applications. <italic<In vivo</italic< efficacy of plasmonic theranostics depends on our ability to monitor temporal changes in their size, shape, and state of aggregation, and the identification of molecules adsorbed on their surfaces. We develop a technique which combines plasmonic and Raman scattering microspectroscopy to colocalize plasmonic scattering from metallic nanoparticles with the Raman signatures of biomolecules adsorbed on the surface of the former. Using this technique, we have colocalized biomolecules with the plasmonic scattering from silver nanoparticles in the vicinity of <italic<Escherichia coli</italic< bacteria. To prove the applicability of this setup for the measurements on mammalian cells, imaging of HEK293 cells treated with gold nanoparticles was performed. We discuss the importance of such correlated measurements over individual techniques, although the latter may lead to misinterpretation of results. Finally, with the above-mentioned examples, we have given criteria to improve the specificity of theranostics. We believe that this methodology will be considered as a prime development in the assessment of theranostics.

Paper Details

Date Published: 22 April 2015
PDF: 7 pages
J. Biomed. Opt. 20(4) 046011 doi: 10.1117/1.JBO.20.4.046011
Published in: Journal of Biomedical Optics Volume 20, Issue 4
Show Author Affiliations
Kamalesh Chaudhari, Indian Institute of Technology Madras (India)
Thalappil Pradeep, Indian Institute of Technology Madras (India)


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