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Journal of Biomedical Optics • Open Access

Labeling of HeLa cells using ZrO2:Yb3+-Er3+ nanoparticles with upconversion emission

Paper Abstract

This work reports the synthesis, structural characterization, and optical properties of ZrO2:Yb3+-Er3+ (2–1 mol%) nanocrystals. The nanoparticles were coated with 3-aminopropyl triethoxysilane (APTES) and further modified with biomolecules, such as Biotin-Anti-rabbit (mouse IgG) and rabbit antibody-AntiKi-67, through a conjugation method. The conjugation was successfully confirmed by Fourier transform infrared, zeta potential, and dynamic light scattering. The internalization of the conjugated nanoparticles in human cervical cancer (HeLa) cells was followed by two-photon confocal microscopy. The ZrO2:Yb3+-Er3+ nanocrystals exhibited strong red emission under 970-nm excitation. Moreover, the luminescence change due to the addition of APTES molecules and biomolecules on the nanocrystals was also studied. These results demonstrate that ZrO2:Yb3+-Er3+ nanocrystals can be successfully functionalized with biomolecules to develop platforms for biolabeling and bioimaging.

Paper Details

Date Published: 16 April 2015
PDF: 8 pages
J. Biomed. Opt. 20(4) 046006 doi: 10.1117/1.JBO.20.4.046006
Published in: Journal of Biomedical Optics Volume 20, Issue 4
Show Author Affiliations
Andrea Ceja-Fdez, Ctr. de Investigaciones en Óptica, A.C. (México)
Tzarara López-Luke, Ctr. de Investigaciones en Óptica, A.C. (México)
Jorge Oliva, Ctr. de Investigaciones Ópticas (México)
Juan Vivero-Escoto, The Univ. of North Carolina at Charlotte (United States)
Ana Lilia Gonzalez-Yebra, Univ. de Guanajuato (México)
Ruben A. Rodríguez-Rojas, Univ. de Guadalajara (México)
Andrea Martínez-Pérez, Ctr. de Investigaciones en Óptica, A.C. (Mexico)
Univ. de Guadalajara (Mexico)
Elder de la Rosa, Ctr. de Investigaciones en Óptica, A.C. (México)

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