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Journal of Biomedical Optics • Open Access

Quantification of in vitro mesenchymal stem cell invasion into tumor spheroids using selective plane illumination microscopy
Author(s): Svenja Rühland; Alexandra Wechselberger; Christine Spitzweg; Ralf Huss; Peter J. Nelson; Hartmann Harz

Paper Abstract

Mesenchymal stem cell (MSC) homing and integration into tumors are under evaluation for clinical application. This approach requires the identification of conditions for optimal tumor invasion. We describe a tool for the <italic<in vitro</italic< comparison of parameters influencing invasion. Human MSC added to experimental tumor spheroids variably migrates toward the center of the structure. To determine MSC distribution inside the three-dimensional specimen, spatial analysis was performed using selective plane illumination microscopy. A standardized method to quantify and compare the invasion potential of variably treated MSC into experimental tumor environments allows efficient screening for optimizing conditions.

Paper Details

Date Published: 3 April 2015
PDF: 3 pages
J. Biomed. Opt. 20(4) 040501 doi: 10.1117/1.JBO.20.4.040501
Published in: Journal of Biomedical Optics Volume 20, Issue 4
Show Author Affiliations
Svenja Rühland, Ludwig-Maximilians-Univ. München (Germany)
Alexandra Wechselberger, Ludwig-Maximilians-Univ. München (Germany)
Christine Spitzweg, Ludwig-Maximilians-Univ. München (Germany)
Ralf Huss, apceth GmbH & Co. KG (Germany)
Peter J. Nelson, Ludwig-Maximilians-Univ. München (Germany)
Hartmann Harz, Ludwig-Maximilians-Univ. München (Germany)


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