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Journal of Biomedical Optics

Label-free detection and characterization of the binding of hemagglutinin protein and broadly neutralizing monoclonal antibodies using terahertz spectroscopy
Author(s): Yiwen Sun; Junlan Zhong; Cunlin Zhang; Jian Zuo; Emma Pickwell-MacPherson
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Paper Abstract

Hemagglutinin (HA) is the main surface glycoprotein of the influenza A virus. The H9N2 subtype influenza A virus is recognized as the most possible pandemic strain as it has crossed the species barrier, infecting swine and humans. We use terahertz spectroscopy to study the hydration shell formation around H9 subtype influenza A virus’s HA protein (H9 HA) as well as the detection of antigen binding of H9 HA with the broadly neutralizing monoclonal antibody. We observe a remarkable concentration dependent nonlinear response of the H9 HA, which reveals the formation process of the hydration shell around H9 HA molecules. Furthermore, we show that terahertz dielectric properties of the H9 HA are strongly affected by the presence of the monoclonal antibody F10 and that the terahertz dielectric loss tangent can be used to detect the antibody binding at lower concentrations than the standard ELISA test.

Paper Details

Date Published: 10 March 2015
PDF: 6 pages
J. Biomed. Opt. 20(3) 037006 doi: 10.1117/1.JBO.20.3.037006
Published in: Journal of Biomedical Optics Volume 20, Issue 3
Show Author Affiliations
Yiwen Sun, Shenzhen Univ. (China)
Junlan Zhong, Shenzhen Univ. (China)
Cunlin Zhang, Capital Normal Univ. (China)
Jian Zuo, Capital Normal Univ. (China)
Emma Pickwell-MacPherson, Hong Kong Univ. of Science and Technology (Hong Kong)

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