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Journal of Biomedical Optics

Macroscopic-imaging technique for subsurface quantification of near-infrared markers during surgery
Author(s): Michael Jermyn; Kolbein K. Kolste; Julien Pichette; Guillaume Sheehy; Leticia M. Angulo-Rodríguez; Keith D. Paulsen; David W. Roberts; Brian C. Wilson; Kevin Petrecca; Frederic Leblond
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Paper Abstract

Obtaining accurate quantitative information on the concentration and distribution of fluorescent markers lying at a depth below the surface of optically turbid media, such as tissue, is a significant challenge. Here, we introduce a fluorescence reconstruction technique based on a diffusion light transport model that can be used during surgery, including guiding resection of brain tumors, for depth-resolved quantitative imaging of near-infrared fluorescent markers. Hyperspectral fluorescence images are used to compute a topographic map of the fluorophore distribution, which yields structural and optical constraints for a three-dimensional subsequent hyperspectral diffuse fluorescence reconstruction algorithm. Using the model fluorophore Alexa Fluor 647 and brain-like tissue phantoms, the technique yielded estimates of fluorophore concentration within ±25% of the true value to depths of 5 to 9 mm, depending on the concentration. The approach is practical for integration into a neurosurgical fluorescence microscope and has potential to further extend fluorescence-guided resection using objective and quantified metrics of the presence of residual tumor tissue.

Paper Details

Date Published: 20 March 2015
PDF: 9 pages
J. Biomed. Opt. 20(3) 036014 doi: 10.1117/1.JBO.20.3.036014
Published in: Journal of Biomedical Optics Volume 20, Issue 3
Show Author Affiliations
Michael Jermyn, McGill Univ. (Canada)
Ecole Polytechnique de Montréal (Canada)
Kolbein K. Kolste, Thayer School of Engineering at Dartmouth (United States)
Julien Pichette, Ecole Polytechnique de Montréal (Canada)
Guillaume Sheehy, Ecole Polytechnique de Montréal (Canada)
Leticia M. Angulo-Rodríguez, Ecole Polytechnique de Montréal (Canada)
Keith D. Paulsen, Thayer School of Engineering at Dartmouth (United States)
David W. Roberts, Dartmouth Hitchcock Medical Ctr. (United States)
Brian C. Wilson, Univ. Health Network (Canada)
Kevin Petrecca, McGill Univ. (Canada)
Frederic Leblond, Ecole Polytechnique de Montréal (Canada)

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