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Journal of Biomedical Optics

Ultrasound-modulated fluorescence based on donor-acceptor-labeled microbubbles
Author(s): Yuan Liu; Jameel A. Feshitan; Ming-Yuan Wei; Mark A. Borden; Baohong Yuan
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Paper Abstract

A fluorescence resonance energy transfer (FRET)-based microbubble contrast agent system was designed to experimentally demonstrate the concept of ultrasound-modulated fluorescence (UMF). Microbubbles were simultaneously labeled with donor and acceptor fluorophores on the surface to minimize self-quenching and maximize FRET. In response to ultrasound, the quenching efficiency was greatly modulated by changing the distance between the donor and acceptor molecules through microbubble size oscillations. Both donors and acceptors exhibited UMF on individual microbubbles. The UMF strength of the donor was more significant compared to that of the acceptor. Furthermore, the UMF of the donor was observed from a microbubble solution in a turbid media. This study exploits the feasibility of donor–acceptor labeled microbubbles as UMF contrast agents.

Paper Details

Date Published: 19 March 2015
PDF: 8 pages
J. Biomed. Opt. 20(3) 036012 doi: 10.1117/1.JBO.20.3.036012
Published in: Journal of Biomedical Optics Volume 20, Issue 3
Show Author Affiliations
Yuan Liu, The Univ. of Texas at Arlington (United States)
The Univ. of Texas Southwestern Medical Ctr. at Dallas (United States)
Jameel A. Feshitan, Univ. of Colorado (United States)
Ming-Yuan Wei, The Univ. of Texas at Arlington (United States)
The Univ. of Texas Southwestern Medical Ctr. at Dallas (United States)
Mark A. Borden, Univ. of Colorado at Boulder (United States)
Baohong Yuan, The Univ. of Texas at Arlington (United States)
The Univ. of Texas Southwestern Medical Ctr. at Dallas (United States)


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