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Journal of Biomedical Optics

5-aminolevulinic acid induced protoporphyrin IX as a fluorescence marker for quantitative image analysis of high-grade dysplasia in Barrett’s esophagus cellular models
Author(s): Shu-Chi A. Yeh; Samir Sahli; David W. Andrews; Michael S. Patterson; David Armstrong; John Provias; Qiyin Fang
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Paper Abstract

Early detection and treatment of high-grade dysplasia (HGD) in Barrett’s esophagus may reduce the risk of developing esophageal adenocarcinoma. Confocal endomicroscopy (CLE) has shown advantages over routine white-light endoscopic surveillance with biopsy for histological examination; however, CLE is compromised by insufficient contrast and by intra- and interobserver variation. An FDA-approved PDT photosensitizer was used here to reveal morphological and textural features similar to those found in histological analysis. Support vector machines were trained using the aforementioned features to obtain an automatic and robust detection of HGD. Our results showed 95% sensitivity and 87% specificity using the optimal feature combination and demonstrated the potential for extension to a three-dimensional cell model.

Paper Details

Date Published: 19 March 2015
PDF: 10 pages
J. Biomed. Opt. 20(3) 036010 doi: 10.1117/1.JBO.20.3.036010
Published in: Journal of Biomedical Optics Volume 20, Issue 3
Show Author Affiliations
Shu-Chi A. Yeh, McMaster Univ. (Canada)
Samir Sahli, McMaster Univ. (Canada)
David W. Andrews, Sunnybrook Research Institute (Canada)
Michael S. Patterson, McMaster Univ. (Canada)
David Armstrong, McMaster Univ. (Canada)
John Provias, McMaster Univ. (Canada)
Qiyin Fang, McMaster Univ. (Canada)

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