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Journal of Biomedical Optics

Noninvasive photoacoustic microscopy of methemoglobin <italic<in vivo</italic<
Author(s): Min Tang; Yong Zhou; Ruiying Zhang; Lihong V. Wang
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Paper Abstract

Due to the various causes of methemoglobinemia and its potential to be confused with other diseases, <italic<in vivo</italic< measurements of methemoglobin have significant applications in the clinic. Using photoacoustic microscopy (PAM), we quantified the average and the distributed percentage of methemoglobin both <italic<in vitro</italic< and <italic<in vivo</italic<. Based on the absorption spectra of methemoglobin, oxyhemoglobin, and deoxyhemoglobin, three wavelengths were chosen to differentiate methemoglobin from the others. The methemoglobin concentrations calculated from the photoacoustic signals agreed well with the preset concentrations. Then we imaged the methemoglobin percentage in microtubes that mimicked blood vessels. Average percentages calculated for five samples with different methemoglobin concentrations also agreed well with the preset values. Finally, we demonstrated the ability of PAM to detect methemoglobin <italic<in vivo</italic< in a mouse ear. Our results show that PAM can quantitatively image methemoglobin distribution <italic<in vivo</italic<.

Paper Details

Date Published: 11 March 2015
PDF: 6 pages
J. Biomed. Opt. 20(3) 036007 doi: 10.1117/1.JBO.20.3.036007
Published in: Journal of Biomedical Optics Volume 20, Issue 3
Show Author Affiliations
Min Tang, Washington Univ. in St. Louis (United States)
Yong Zhou, Washington Univ. in St. Louis (United States)
Ruiying Zhang, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)

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