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Journal of Biomedical Optics • Open Access

Visible micro-Raman spectroscopy of single human mammary epithelial cells exposed to x-ray radiation
Author(s): Ines Delfino; Giuseppe Perna; Maria Lasalvia; Vito Capozzi; Lorenzo Manti; Carlo Camerlingo; Maria Lepore

Paper Abstract

A micro-Raman spectroscopy investigation has been performed in vitro on single human mammary epithelial cells after irradiation by graded x-ray doses. The analysis by principal component analysis (PCA) and interval-PCA (i-PCA) methods has allowed us to point out the small differences in the Raman spectra induced by irradiation. This experimental approach has enabled us to delineate radiation-induced changes in protein, nucleic acid, lipid, and carbohydrate content. In particular, the dose dependence of PCA and i-PCA components has been analyzed. Our results have confirmed that micro-Raman spectroscopy coupled to properly chosen data analysis methods is a very sensitive technique to detect early molecular changes at the single-cell level following exposure to ionizing radiation. This would help in developing innovative approaches to monitor radiation cancer radiotherapy outcome so as to reduce the overall radiation dose and minimize damage to the surrounding healthy cells, both aspects being of great importance in the field of radiation therapy.

Paper Details

Date Published: 13 March 2015
PDF: 13 pages
J. Biomed. Opt. 20(3) 035003 doi: 10.1117/1.JBO.20.3.035003
Published in: Journal of Biomedical Optics Volume 20, Issue 3
Show Author Affiliations
Ines Delfino, Univ. degli Studi della Tuscia (Italy)
Giuseppe Perna, Univ. degli Studi di Foggia (Italy)
Maria Lasalvia, Univ. degli Studi di Foggia (Italy)
Vito Capozzi, Univ. degli Studi di Foggia (Italy)
Lorenzo Manti, Univ. degli Studi di Napoli Federico II (Italy)
Carlo Camerlingo, Consiglio Nazionale delle Ricerche (Italy)
Maria Lepore, Seconda Univ. degli Studi di Napoli (Italy)

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