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Journal of Biomedical Optics • Open Access

Quantitative optical coherence tomography imaging of intermediate flow defect phenotypes in ciliary physiology and pathophysiology
Author(s): Brendan K. Huang; Ute A. Gamm; Stephan Jonas; Mustafa K. Khokha; Michael A. Choma

Paper Abstract

Cilia-driven fluid flow is a critical yet poorly understood aspect of pulmonary physiology. Here, we demonstrate that optical coherence tomography-based particle tracking velocimetry can be used to quantify subtle variability in cilia-driven flow performance in Xenopus, an important animal model of ciliary biology. Changes in flow performance were quantified in the setting of normal development, as well as in response to three types of perturbations: mechanical (increased fluid viscosity), pharmacological (disrupted serotonin signaling), and genetic (diminished ciliary motor protein expression). Of note, we demonstrate decreased flow secondary to gene knockdown of kif3a a protein involved in ciliogenesis, as well as a dose-response decrease in flow secondary to knockdown of dnah9, an important ciliary motor protein.

Paper Details

Date Published: 9 March 2015
PDF: 3 pages
J. Biomed. Opt. 20(3) 030502 doi: 10.1117/1.JBO.20.3.030502
Published in: Journal of Biomedical Optics Volume 20, Issue 3
Show Author Affiliations
Brendan K. Huang, Yale School of Medicine (United States)
Ute A. Gamm, Yale School of Medicine (United States)
Stephan Jonas, Yale School of Medicine (United States)
Mustafa K. Khokha, Yale School of Medicine (United States)
Michael A. Choma, Yale School of Medicine (United States)

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