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Journal of Biomedical Optics

Antibacterial photodynamic therapy with 808-nm laser and indocyanine green on abrasion wound models
Author(s): Nermin Topaloglu; Melike Güney; Sahru Yuksel; Murat Gülsoy
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Paper Abstract

Infections with pathogens could cause serious health problems, such as septicemia and subsequent death. Some of these deaths are caused by nosocomial, chronic, or burn-related wound infections. Photodynamic therapy (PDT) can be useful for the treatment of these infections. Our aim was to investigate the antibacterial effect of indocyanine green (ICG) and 808-nm laser on a rat abrasion wound model infected with the multidrug resistant Staphylococcus aureus strain. Abrasion wounds were infected with a multidrug resistant clinical isolate of S. aureus. ICG concentrations of 500, 1000, and 2000  μg/ml were applied with a 450  J/cm2 energy dose. Temperature change was monitored by a thermocouple system. The remaining bacterial burden was determined by the serial dilution method after each application. Wounds were observed for 11 days posttreatment. The recovery process was assessed macroscopically. Tissue samples were also examined histologically by hematoxylin–eosin staining. Around a 90% reduction in bacterial burden was observed after PDT applications. In positive control groups (ICG-only and laser-only groups), there was no significant reduction. The applied energy dose did not cause any thermal damage to the target tissue or host environment. Results showed that ICG together with a 808-nm laser might be a promising antibacterial method to eliminate infections in animals and accelerate the wound-healing process.

Paper Details

Date Published: 18 February 2015
PDF: 7 pages
J. Biomed. Opt. 20(2) 028003 doi: 10.1117/1.JBO.20.2.028003
Published in: Journal of Biomedical Optics Volume 20, Issue 2
Show Author Affiliations
Nermin Topaloglu, Bogaziçi Univ. (Turkey)
Melike Güney, Bogaziçi Üniv. (Turkey)
Sahru Yuksel, Bogaziçi Üniv. (Turkey)
Murat Gülsoy, Bogaziçi Univ. (Turkey)


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