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Journal of Biomedical Optics • Open Access

5-aminolevulinic acid for quantitative seek-and-treat of high-grade dysplasia in Barrett’s esophagus cellular models
Author(s): Shu-Chi A. Yeh; Celine Ling; David W. Andrews; Michael S. Patterson; Kevin R. Diamond; Joseph E. Hayward; David Armstrong; Qiyin Fang

Paper Abstract

High-grade dysplasia (HGD) in Barrett’s esophagus (BE) poses increased risk for developing esophageal adenocarcinoma. To date, early detection and treatment of HGD regions are still challenging due to the sampling error from tissue biopsy and relocation error during the treatment after histopathological analysis. In this study, CP-A (metaplasia) and CP-B (HGD) cell lines were used to investigate the “seek-and-treat” potential using 5-aminolevulinic acid-induced protoporphyrin IX (PpIX). The photodynamic therapy photosensitizer then provides both a phototoxic effect and additional image contrast for automatic detection and real-time laser treatment. Complementary to our studies on automatic classification, this work focused on characterizing subcellular irradiation and the potential phototoxicity on both metaplasia and HGD. The treatment results showed that the HGD cells are less viable than metaplastic cells due to more PpIX production at earlier times. Also, due to mitochondrial localization of PpIX, a better killing effect was achieved by involving mitochondria or whole cells compared with just nucleus irradiation in the detected region. With the additional toxicity given by PpIX and potential morphological/textural differences for pattern recognition, this cellular platform serves as a platform to further investigate real-time “seek-and-treat” strategies in three-dimensional models for improving early detection and treatment of BE.

Paper Details

Date Published: 11 February 2015
PDF: 8 pages
J. Biomed. Opt. 20(2) 028002 doi: 10.1117/1.JBO.20.2.028002
Published in: Journal of Biomedical Optics Volume 20, Issue 2
Show Author Affiliations
Shu-Chi A. Yeh, McMaster Univ. (Canada)
Celine Ling, McMaster Univ. (Canada)
David W. Andrews, Sunnybrook Research Institute (Canada)
Michael S. Patterson, McMaster Univ. (Canada)
Kevin R. Diamond, McMaster Univ. (Canada)
Joseph E. Hayward, McMaster Univ. (Canada)
David Armstrong, McMaster Univ. (Canada)
Qiyin Fang, McMaster Univ. (Canada)

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