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Journal of Biomedical Optics

Tip-enhanced fluorescence with radially polarized illumination for monitoring loop-mediated isothermal amplification on Hepatitis C virus cDNA
Author(s): Shih-Chung Wei; Tsung-Liang Chuang; Da-Shin Wang; Hui-Hsin Lu; Frank X. Gu; Kung-Bin Sung; Chii-Wann Lin
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Paper Abstract

A tip nanobiosensor for monitoring DNA replication was presented. The effects of excitation power and polarization on tip-enhanced fluorescence (TEF) were assessed with the tip immersed in fluorescein isothiocyanate solution first. The photon count rose on average fivefold with radially polarized illumination at 50 mW. We then used polymerase-functionalized tips for monitoring loop-mediated isothermal amplification on Hepatitis C virus cDNA. The amplicon-SYBR® Green I complex was detected and compared to real-time loop-mediated isothermal amplification. The signals of the reaction using 4 and 0.004  ng/μl templates were detected 10 and 30 min earlier, respectively. The results showed the potential of TEF in developing a nanobiosensor for real-time DNA amplification.

Paper Details

Date Published: 13 February 2015
PDF: 7 pages
J. Biomed. Opt. 20(2) 027005 doi: 10.1117/1.JBO.20.2.027005
Published in: Journal of Biomedical Optics Volume 20, Issue 2
Show Author Affiliations
Shih-Chung Wei, National Taiwan Univ. (Taiwan)
Tsung-Liang Chuang, National Taiwan Univ. (Taiwan)
Da-Shin Wang, National Taiwan Univ. (Taiwan)
Hui-Hsin Lu, National Taiwan Univ. (Taiwan)
Frank X. Gu, Univ. of Waterloo (Canada)
Kung-Bin Sung, National Taiwan Univ. (Taiwan)
National Taiwan University (Taiwan)
Chii-Wann Lin, National Taiwan Univ. (Taiwan)
National Taiwan University (Taiwan)

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