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Journal of Biomedical Optics • Open Access

Label-free nonenzymatic glycation monitoring of collagen scaffolds in type 2 diabetic mice by confocal Raman microspectroscopy
Author(s): Panpan Shi; Hanping Liu; Xiaoyuan Deng; Ying Jin; Qiannan Wang; Hao Liu; Maosheng Chen; Xue Han

Paper Abstract

Collagen is the key target of nonenzymatic glycation during physiopathological processes such as diabetes. The induced changes in the biochemical property of collagen by nonenzymatic glycation remain a major challenge to probe. This study investigated the use of confocal Raman microspectroscopy to label-free monitor the nonenzymatic glycation of collagen scaffolds from type 2 diabetic (T2D) mice at different timepoints (0, 4, 8, and 12 weeks). The glycated collagen scaffolds were obtained through the decellularized dermal matrix method to remove the epidermis layer, subcutaneous tissue, and cells in the dermis and to retain the collagen fibrils. Raman spectra showed no changes in Raman peak positions, which indicated that nonenzymatic glycation could produce no significant changes in the triple-helix structure of collagen in T2D mice. However, the relative intensity of the Raman bands at 921, 1033, 1244, 1274, 1346, 1635, and 1672  cm−1 increased as diabetic time progressed. Correlation analysis suggested that the spectra of these bands had a high positive correlation with the expression of anti-advanced glycation end products obtained by immunofluorescence imaging of the same collagen scaffolds. Confocal Raman microspectroscopy proves a potential tool to label-free monitor the collagen changes caused by nonenzymatic glycation in T2D mice.

Paper Details

Date Published: 11 February 2015
PDF: 9 pages
J. Biomed. Opt. 20(2) 027002 doi: 10.1117/1.JBO.20.2.027002
Published in: Journal of Biomedical Optics Volume 20, Issue 2
Show Author Affiliations
Panpan Shi, South China Normal Univ. (China)
Hanping Liu, South China Normal Univ. (China)
Xiaoyuan Deng, South China Normal Univ. (China)
Ying Jin, South China Normal Univ. (China)
Qiannan Wang, South China Normal Univ. (China)
Hao Liu, South China Normal Univ. (China)
Maosheng Chen, South China Normal Univ. (China)
Xue Han, South China Normal Univ. (China)

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