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Journal of Biomedical Optics

Enhanced resolution through thick tissue with structured illumination and adaptive optics
Author(s): Benjamin Thomas; Adrian Wolstenholme; Snehal N. Chaudhari; Edward T. Kipreos; Peter Kner
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Paper Abstract

Structured illumination microscopy provides twice the linear resolution of conventional fluorescence microscopy, but in thick samples, aberrations degrade the performance and limit the resolution. Here, we demonstrate structured illumination microscopy through 35 µm of tissue using adaptive optics (AO) to correct aberrations resulting in images with a resolution of 140 nm. We report a 60% minimum improvement in the signal-to-noise ratio of the structured illumination reconstruction through thick tissue by correction with AO.

Paper Details

Date Published: 25 February 2015
PDF: 8 pages
J. Biomed. Opt. 20(2) 026006 doi: 10.1117/1.JBO.20.2.026006
Published in: Journal of Biomedical Optics Volume 20, Issue 2
Show Author Affiliations
Benjamin Thomas, The Univ. of Georgia (United States)
Adrian Wolstenholme, The Univ. of Georgia (United States)
Snehal N. Chaudhari, The Univ. of Georgia (United States)
Edward T. Kipreos, The Univ. of Georgia (United States)
Peter Kner, The Univ. of Georgia (United States)

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