Share Email Print

Journal of Biomedical Optics

Intravital live cell triggered imaging system reveals monocyte patrolling and macrophage migration in atherosclerotic arteries
Author(s): Sara McArdle; Grzegorz Chodaczek; Nilanjan Ray; Klaus Ley
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Intravital multiphoton imaging of arteries is technically challenging because the artery expands with every heartbeat, causing severe motion artifacts. To study leukocyte activity in atherosclerosis, we developed the intravital live cell triggered imaging system (ILTIS). This system implements cardiac triggered acquisition as well as frame selection and image registration algorithms to produce stable movies of myeloid cell movement in atherosclerotic arteries in live mice. To minimize tissue damage, no mechanical stabilization is used and the artery is allowed to expand freely. ILTIS performs multicolor high frame-rate two-dimensional imaging and full-thickness three-dimensional imaging of beating arteries in live mice. The external carotid artery and its branches (superior thyroid and ascending pharyngeal arteries) were developed as a surgically accessible and reliable model of atherosclerosis. We use ILTIS to demonstrate <italic<Cx3cr1</italic<<italic< GFP </italic< monocytes patrolling the lumen of atherosclerotic arteries. Additionally, we developed a new reporter mouse (<italic<Apoe</italic<<italic< −/− </italic<<italic<Cx3cr1</italic<<italic< GFP/+ </italic<<italic<Cd11c</italic<<italic< YFP </italic<) to image GFP+ and GFP+YFP+ macrophages “dancing on the spot” and YFP+ macrophages migrating within intimal plaque. ILTIS will be helpful to answer pertinent open questions in the field, including monocyte recruitment and transmigration, macrophage and dendritic cell activity, and motion of other immune cells.

Paper Details

Date Published: 24 February 2015
PDF: 9 pages
J. Biomed. Opt. 20(2) 026005 doi: 10.1117/1.JBO.20.2.026005
Published in: Journal of Biomedical Optics Volume 20, Issue 2
Show Author Affiliations
Sara McArdle, La Jolla Institute for Allergy and Immunology (United States)
Univ. of California, San Diego (United States)
Grzegorz Chodaczek, La Jolla Institute for Allergy and Immunology (United States)
Wroclaw Research Centre EIT+ (Poland)
Nilanjan Ray, Univ. of Alberta (Canada)
Klaus Ley, La Jolla Insitute for Allergy and Immunology (United States)

© SPIE. Terms of Use
Back to Top