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Journal of Biomedical Optics

Optical properties of rabbit brain in the red and near-infrared: changes observed under in vivo, postmortem, frozen, and formalin-fixated conditions
Author(s): Andreas Pitzschke; Blaise Lovisa; Olivier Seydoux; Matthias Haenggi; Markus F. Oertel; Matthieu Zellweger; Yanik Tardy; Georges A. Wagnières
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Paper Abstract

The outcome of light-based therapeutic approaches depends on light propagation in biological tissues, which is governed by their optical properties. The objective of this study was to quantify optical properties of brain tissue in vivo and postmortem and assess changes due to tissue handling postmortem. The study was carried out on eight female New Zealand white rabbits. The local fluence rate was measured in the VIS/NIR range in the brain in vivo, just postmortem, and after six weeks’ storage of the head at −20°C or in 10% formaldehyde solution. Only minimal changes in the effective attenuation coefficient μeff were observed for two methods of sacrifice, exsanguination or injection of KCl. Under all tissue conditions, μeff decreased with increasing wavelengths. After long-term storage for six weeks at −20°C, μeff decreased, on average, by 15 to 25% at all wavelengths, while it increased by 5 to 15% at all wavelengths after storage in formaldehyde. We demonstrated that μeff was not very sensitive to the method of animal sacrifice, that tissue freezing significantly altered tissue optical properties, and that formalin fixation might affect the tissue’s optical properties.

Paper Details

Date Published: 23 February 2015
PDF: 9 pages
J. Biomed. Opt. 20(2) 025006 doi: 10.1117/1.JBO.20.2.025006
Published in: Journal of Biomedical Optics Volume 20, Issue 2
Show Author Affiliations
Andreas Pitzschke, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Blaise Lovisa, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Medos International Sàrl (Switzerland)
Olivier Seydoux, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Matthias Haenggi, Inselspital Bern (Switzerland)
Markus F. Oertel, Inselspital Bern (Switzerland)
Matthieu Zellweger, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Yanik Tardy, Medos International Sàrl (Switzerland)
Georges A. Wagnières, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

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