Share Email Print

Journal of Biomedical Optics

Experimental recovery of intrinsic fluorescence and fluorophore concentration in the presence of hemoglobin: spectral effect of scattering and absorption on fluorescence
Author(s): Vinh Nguyen Du Le; Michael S. Patterson; Thomas J. Farrell; Joseph E. Hayward; Qiyin Fang
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The ability to recover the intrinsic fluorescence of biological fluorophores is crucial to accurately identify the fluorophores and quantify their concentrations in the media. Although some studies have successfully retrieved the fluorescence spectral shape of known fluorophores, the techniques usually came with heavy computation costs and did not apply for strongly absorptive media, and the intrinsic fluorescence intensity and fluorophore concentration were not recovered. In this communication, an experimental approach was presented to recover intrinsic fluorescence and concentration of fluorescein in the presence of hemoglobin (Hb). The results indicated that the method was efficient in recovering the intrinsic fluorescence peak and fluorophore concentration with an error of 3% and 10%, respectively. The results also suggested that chromophores with irregular absorption spectra (e.g., Hb) have more profound effects on fluorescence spectral shape than chromophores with monotonic absorption and scattering spectra (e.g., black India ink and polystyrene microspheres).

Paper Details

Date Published: 22 December 2015
PDF: 10 pages
J. Biomed. Opt. 20(12) 127003 doi: 10.1117/1.JBO.20.12.127003
Published in: Journal of Biomedical Optics Volume 20, Issue 12
Show Author Affiliations
Vinh Nguyen Du Le, McMaster Univ. (Canada)
Michael S. Patterson, McMaster Univ. (Canada)
Juravinski Cancer Ctr. (Canada)
Thomas J. Farrell, McMaster Univ. (Canada)
Juravinski Cancer Ctr. (Canada)
Joseph E. Hayward, McMaster Univ. (Canada)
Juravinski Cancer Ctr. (Canada)
Qiyin Fang, McMaster Univ. (Canada)

© SPIE. Terms of Use
Back to Top