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Journal of Biomedical Optics

Living cell dry mass measurement using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion
Author(s): Sherazade Aknoun; Julien Savatier; Pierre Bon; Frédéric Galland; Lamiae Abdeladim; Benoit F. Wattellier; Serge Monneret
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Paper Abstract

Single-cell dry mass measurement is used in biology to follow cell cycle, to address effects of drugs, or to investigate cell metabolism. Quantitative phase imaging technique with quadriwave lateral shearing interferometry (QWLSI) allows measuring cell dry mass. The technique is very simple to set up, as it is integrated in a camera-like instrument. It simply plugs onto a standard microscope and uses a white light illumination source. Its working principle is first explained, from image acquisition to automated segmentation algorithm and dry mass quantification. Metrology of the whole process, including its sensitivity, repeatability, reliability, sources of error, over different kinds of samples and under different experimental conditions, is developed. We show that there is no influence of magnification or spatial light coherence on dry mass measurement; effect of defocus is more critical but can be calibrated. As a consequence, QWLSI is a well-suited technique for fast, simple, and reliable cell dry mass study, especially for live cells.

Paper Details

Date Published: 22 December 2015
PDF: 15 pages
J. Biomed. Opt. 20(12) 126009 doi: 10.1117/1.JBO.20.12.126009
Published in: Journal of Biomedical Optics Volume 20, Issue 12
Show Author Affiliations
Sherazade Aknoun, Aix-Marseille Univ. (France)
PHASICS S.A. (France)
Julien Savatier, Aix-Marseille Univ. (France)
Pierre Bon, Aix-Marseille Univ. (France)
Frédéric Galland, Aix-Marseille Univ. (France)
Lamiae Abdeladim, Aix-Marseille Univ. (France)
Benoit F. Wattellier, PHASICS S.A. (France)
Serge Monneret, Aix-Marseille Univ. (France)

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