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Journal of Biomedical Optics • Open Access

In vivo wide-field multispectral scanning laser ophthalmoscopy–optical coherence tomography mouse retinal imager: longitudinal imaging of ganglion cells, microglia, and Müller glia, and mapping of the mouse retinal and choroidal vasculature
Author(s): Pengfei Zhang; Azhar Zam; Yifan Jian; Xinlei Wang; Yuanpei Li; Kit S. Lam; Marie E. Burns; Marinko V. Sarunic; Edward N. Pugh; Robert J. Zawadzki

Paper Abstract

Scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT) provide complementary views of the retina, with the former collecting fluorescence data with good lateral but relatively low-axial resolution, and the latter collecting label-free backscattering data with comparable lateral but much higher axial resolution. To take maximal advantage of the information of both modalities in mouse retinal imaging, we have constructed a compact, four-channel, wide-field (∼50  deg) system that simultaneously acquires and automatically coregisters three channels of confocal SLO and Fourier domain OCT data. The scanner control system allows “zoomed” imaging of a region of interest identified in a wide-field image, providing efficient digital sampling and localization of cellular resolution features in longitudinal imaging of individual mice. The SLO is equipped with a “flip-in” spectrometer that enables spectral “fingerprinting” of fluorochromes. Segmentation of retina layers and en face display facilitate spatial comparison of OCT data with SLO fluorescence patterns. We demonstrate that the system can be used to image an individual retinal ganglion cell over many months, to simultaneously image microglia and Müller glia expressing different fluorochromes, to characterize the distinctive spatial distributions and clearance times of circulating fluorochromes with different molecular sizes, and to produce unequivocal images of the heretofore uncharacterized mouse choroidal vasculature.

Paper Details

Date Published: 16 December 2015
PDF: 10 pages
J. Biomed. Opt. 20(12) 126005 doi: 10.1117/1.JBO.20.12.126005
Published in: Journal of Biomedical Optics Volume 20, Issue 12
Show Author Affiliations
Pengfei Zhang, Univ. of California, Davis (Unites States)
Azhar Zam, Univ. of California, Davis (Unites States)
Yifan Jian, Simon Fraser Univ. (Canada)
Xinlei Wang, Univ. of California, Davis (Unites States)
Yuanpei Li, UC Davis Comprehensive Cancer Ctr. (Unites States)
Kit S. Lam, UC Davis Comprehensive Cancer Ctr. (Unites States)
Marie E. Burns, Univ. of California, Davis (United States)
Marinko V. Sarunic, Simon Fraser Univ. (Canada)
Edward N. Pugh, Univ. of California, Davis (Unites States)
Robert J. Zawadzki, Univ. of California, Davis (United States)

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