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Journal of Biomedical Optics

Optical properties of tumor tissues grown on the chorioallantoic membrane of chicken eggs: tumor model to assay of tumor response to photodynamic therapy
Author(s): Norihiro Honda; Yoichiro Kariyama; Hisanao Hazama; Takuya Ishii; Yuya Kitajima; Katsushi Inoue; Masahiro Ishizuka; Tohru Tanaka; Kunio Awazu
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Paper Abstract

Herein, the optical adequacy of a tumor model prepared with tumor cells grown on the chorioallantoic membrane (CAM) of a chicken egg is evaluated as an alternative to the mouse tumor model to assess the optimal irradiation conditions in photodynamic therapy (PDT). The optical properties of CAM and mouse tumor tissues were measured with a double integrating sphere and the inverse Monte Carlo technique in the 350- to 1000-nm wavelength range. The hemoglobin and water absorption bands observed in the CAM tumor tissue (10 eggs and 10 tumors) are equal to that of the mouse tumor tissue (8 animals and 8 tumors). The optical intersubject variability of the CAM tumor tissues meets or exceeds that of the mouse tumor tissues, and the reduced scattering coefficient spectra of CAM tumor tissues can be equated with those of mouse tumor tissues. These results confirm that the CAM tumor model is a viable alternative to the mouse tumor model, especially for deriving optimal irradiation conditions in PDT.

Paper Details

Date Published: 11 December 2015
PDF: 8 pages
J. Biomed. Opt. 20(12) 125001 doi: 10.1117/1.JBO.20.12.125001
Published in: Journal of Biomedical Optics Volume 20, Issue 12
Show Author Affiliations
Norihiro Honda, Osaka Univ. (Japan)
Yoichiro Kariyama, Osaka Univ. (Japan)
Hisanao Hazama, Osaka Univ. (Japan)
Takuya Ishii, SBI Pharmaceuticals Co., Ltd. (Japan)
Yuya Kitajima, SBI Pharmaceuticals Co., Ltd. (Japan)
Katsushi Inoue, SBI Pharmaceuticals Co., Ltd. (Japan)
Masahiro Ishizuka, SBI Pharmaceuticals Co., Ltd. (Japan)
Tohru Tanaka, SBI Pharmaceuticals Co., Ltd. (Japan)
Kunio Awazu, Osaka Univ. (Japan)

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