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Journal of Biomedical Optics

Rapid and high-resolution imaging of human liver specimens by full-field optical coherence tomography
Author(s): Yue Zhu; Wanrong Gao; Yuan Zhou; Yingcheng Guo; Feng Guo; Yong He
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Paper Abstract

We report rapid and high-resolution tomographic en face imaging of human liver specimens by full-field optical coherence tomography (FF-OCT). First, the arrangement of the FF-OCT system was described and the performance of the system was measured. The measured axial and lateral resolutions of the system are 0.8 and 0.9  μm, respectively. The system has a sensitivity of ∼60  dB and can achieve an imaging rate of 7 fps and a penetration depth of ∼80  μm. The histological structures of normal liver can be seen clearly in the en face tomographic images, including central veins, cords of hepatocytes separated by sinusoidal spaces, and portal area (portal vein, the hepatic arteriole, and the bile duct). A wide variety of histological subtypes of hepatocellular carcinoma was observed in en face tomographic images, revealing notable cancerous features, including the nuclear atypia (enlarged convoluted nuclei), the polygonal tumor cells with obvious resemblance to hepatocytes with enlarged nuclei. In addition, thicker fibrous bands, which make the cytoplasmic plump vesicular nuclei indistinct, were also seen in the images. Finally, comparison between the portal vein in a normal specimen versus that seen in the rare type of cholangiocarcinoma was made. The results show that the cholangiocarcinoma presents with a blurred pattern of portal vein in the lateral direction and an aggregated distribution in the axial direction; the surrounding sinusoidal spaces and nuclei of cholangiocarcinoma are absent. The findings in this work may be used as additional signs of liver cancer or cholangiocarcinoma, demonstrating capacity of FF-OCT device for early cancer diagnosis and many other tumor-related studies in biopsy.

Paper Details

Date Published: 30 November 2015
PDF: 7 pages
J. Biomed. Opt. 20(11) 116010 doi: 10.1117/1.JBO.20.11.116010
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
Yue Zhu, Nanjing Univ. of Science and Technology (China)
Wanrong Gao, Nanjing Univ. of Science and Technology (China)
Yuan Zhou, Nanjing Univ. (China)
Yingcheng Guo, Nanjing Univ. of Science and Technology (China)
Feng Guo, Xi'an Railway Vocational & Technical Institute (China)
Yong He, Nanjing Univ. of Science and Technology (China)

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